p426 GAL

 
LOCUS       p426 GAL	6412 bp 	DNA	SYN
DEFINITION  p426 GAL
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6412
                     /organism="p426 GAL"
                     /mol_type="other DNA"
     misc_feature    complement(29..51)
                     /label="pGEX_3_primer"
     promoter        202..415
                     /label="URA3_promoter"
     gene            417..1217
                     /label="URA3"
                     /gene="URA3"
     CDS             417..1220
                     /label="ORF frame 3"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
     CDS             complement(452..928)
                     /label="ORF frame 1"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     misc_feature    complement(1822..1965)
                     /label="lacZ_a"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     misc_feature    1936..1958
                     /label="M13_pUC_fwd_primer"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     promoter        1951..1967
                     /label="M13_forward20_primer"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     promoter        1977..1995
                     /label="T7_promoter"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     terminator      complement(2021..2260)
                     /label="CYC1_terminator"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     misc_feature    2227..2245
                     /label="pYESTrp_rev_primer"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     misc_feature    2227..2245
                     /label="CYC1_primer"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     misc_feature    2263..2279
                     /label="pBluescriptKS_primer"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     misc_feature    complement(2314..2330)
                     /label="pBluescriptSK_primer"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     promoter        complement(2337..2787)
                     /label="GAL1_promoter"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     misc_feature    complement(2351..2374)
                     /label="GAL1_primer"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     promoter        complement(2809..2828)
                     /label="T3_promoter"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     promoter        complement(2845..2863)
                     /label="M13_reverse_primer"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     misc_feature    complement(2862..2884)
                     /label="M13_pUC_rev_primer"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     gene            complement(4010..4870)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     CDS             complement(4010..4870)
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(4912..4940)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(5965..6012)
                     /label="FRT"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
   61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
  121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
  181 accataccac agcttttcaa ttcaattcat catttttttt ttattctttt ttttgatttc
  241 ggtttctttg aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg
  301 agcacagact tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc
  361 cagtattctt aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt
  421 cgaaagctac atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat
  481 ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca
  541 aggaattact ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg
  601 tggatatctt gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg
  661 ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca
  721 aattgcagta ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac
  781 acggtgtggt gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa
  841 aggaacctag aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg
  901 gagaatatac taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct
  961 ttattgctca aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac
 1021 ccggtgtggg tttagatgac aagggagacg cattgggtca acagtataga accgtggatg
 1081 atgtggtctc tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa
 1141 gggatgctaa ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa
 1201 gatgcggcca gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac
 1261 aaattagagc ttcaatttaa ttatatcagt tattacccta tgcggtgtga aataccgcac
 1321 agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt ttgttaaaat
 1381 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
 1441 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
 1501 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
 1561 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
 1621 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
 1681 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
 1741 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
 1801 gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
 1861 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
 1921 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
 1981 acgactcact atagggcgaa ttgggtaccg gccgcaaatt aaagccttcg agcgtcccaa
 2041 aaccttctca agcaaggttt tcagtataat gttacatgcg tacacgcgtc tgtacagaaa
 2101 aaaaagaaaa atttgaaata taaataacgt tcttaatact aacataacta taaaaaaata
 2161 aatagggacc tagacttcag gttgtctaac tccttccttt tcggttagag cggatgtggg
 2221 gggagggcgt gaatgtaagc gtgacataac taattacatg actcgaggtc gacggtatcg
 2281 ataagcttga tatcgaattc ctgcagcccg ggggatccac tagttctaga atccggggtt
 2341 ttttctcctt gacgttaaag tatagaggta tattaacaat tttttgttga tacttttatt
 2401 acatttgaat aagaagtaat acaaaccgaa aatgttgaaa gtattagtta aagtggttat
 2461 gcagtttttg catttatata tctgttaata gatcaaaaat catcgcttcg ctgattaatt
 2521 accccagaaa taaggctaaa aaactaatcg cattatcatc ctatggttgt taatttgatt
 2581 cgttcatttg aaggtttgtg gggccaggtt actgccaatt tttcctcttc ataaccataa
 2641 aagctagtat tgtagaatct ttattgttcg gagcagtgcg gcgcgaggca catctgcgtt
 2701 tcaggaacgc gaccggtgaa gacgaggacg cacggaggag agtcttcctt cggagggctg
 2761 tcacccgctc ggcggcttct aatccgtact agagctccag cttttgttcc ctttagtgag
 2821 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
 2881 cgctcacaat tccacacaac ataggagccg gaagcataaa gtgtaaagcc tggggtgcct
 2941 aatgagtgag gtaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
 3001 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
 3061 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
 3121 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
 3181 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
 3241 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
 3301 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
 3361 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
 3421 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
 3481 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
 3541 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
 3601 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
 3661 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
 3721 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
 3781 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
 3841 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
 3901 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
 3961 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
 4021 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
 4081 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
 4141 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
 4201 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
 4261 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
 4321 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
 4381 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
 4441 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
 4501 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
 4561 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
 4621 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
 4681 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
 4741 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
 4801 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
 4861 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
 4921 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
 4981 ttccccgaaa agtgccacct gaacgaagca tctgtgcttc attttgtaga acaaaaatgc
 5041 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
 5101 tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt gtaaaacaaa
 5161 aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac
 5221 agaaatgcaa cgcgagagcg ctattttacc aacaaagaat ctatacttct tttttgttct
 5281 acaaaaatgc atcccgagag cgctattttt ctaacaaagc atcttagatt actttttttc
 5341 tcctttgtgc gctctataat gcagtctctt gataactttt tgcactgtag gtccgttaag
 5401 gttagaagaa ggctactttg gtgtctattt tctcttccat aaaaaaagcc tgactccact
 5461 tcccgcgttt actgattact agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc
 5521 ccgattatat tctataccga tgtggattgc gcatactttg tgaacagaaa gtgatagcgt
 5581 tgatgattct tcattggtca gaaaattatg aacggtttct tctattttgt ctctatatac
 5641 tacgtatagg aaatgtttac attttcgtat tgttttcgat tcactctatg aatagttctt
 5701 actacaattt ttttgtctaa agagtaatac tagagataaa cataaaaaat gtagaggtcg
 5761 agtttagatg caagttcaag gagcgaaagg tggatgggta ggttatatag ggatatagca
 5821 cagagatata tagcaaagag atacttttga gcaatgtttg tggaagcggt attcgcaata
 5881 ttttagtagc tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc
 5941 gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt ctagagaata ggaacttcgg
 6001 aataggaact tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg
 6061 cacatacagc tcactgttca cgtcgcacct atatctgcgt gttgcctgta tatatatata
 6121 catgagaaga acggcatagt gcgtgtttat gcttaaatgc gtacttatat gcgtctattt
 6181 atgtaggatg aaaggtagtc tagtacctcc tgtgatatta tcccattcca tgcggggtat
 6241 cgtatgcttc cttcagcact accctttagc tgttctatat gctgccactc ctcaattgga
 6301 ttagtctcat ccttcaatgc tatcatttcc tttgatattg gatcatacta agaaaccatt
 6361 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc

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